Inhibition of respiration in submitochondrial particles by uncouplers of oxidative phosphorylation
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منابع مشابه
Inhibition of respiration in submitochondrial particles by uncouplers of oxidative phosphorylation.
High concentrations of uncouplers of oxidative phosphorylation inhibit the oxidation of succinate and NADH catalyzed by phosphorylating beef heart submitochondrial particles. Inhibition is reversed by bovine serum albumin but not by ATP. ATP does not influence either the stimulation of respiration at low uncoupler concentrations or the inhibition of respiration at high uncoupler concentrations....
متن کاملUncouplers of oxidative phosphorylation.
Uncouplers of oxidative phosphorylation in mitochondria inhibit the coupling between the electron transport and phosphorylation reactions and thus inhibit ATP synthesis without affecting the respiratory chain and ATP synthase (H(+)-ATPase). Miscellaneous compounds are known to be uncouplers, but weakly acidic uncouplers are representative because they show very potent activities. The most poten...
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was proportional to the acidity of the imidazole -NH group. Tetrachloro-2trifluoromethylbenzimidazole was the most active (50% uncoupling of oxidative phosphorylation at 7-9 x 10-8M, pK5.04). Nitro-substituted analogues were less active than predicted from pK considerations or from partition-coefficient measurements. 3. Introduction of an -NH2 or -CO2H substituent caused a loss of uncoupling ac...
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Addition of several N-arylaminonaphthalenesulfonate dyes to submitochondrial particles from bovine heart resulted in an enhancement of fluorescence of from SOto 150-fold and a considerable shift in the wave length of maximum emission toward the ultraviolet. The results indicate 40 to 55 dyebinding sites per mg of submitochondrial particles. A preliminary search revealed that mitochondrial ATPas...
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1. The maximal H+: 0 ratios of proton transport in highly resolved submitochondrial particles prepared by sonication of bovine heart mitochondria in the presence of ammonia and exposed sequentially to Sephadex and urea (ASU particles) in the presence of oligomycin were found to be 6,4, and 2 during the oxidation of NADH, succinate, or N-methylphenazonium methosulfate, respectively. A pH depende...
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ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 1970
ISSN: 0003-9861
DOI: 10.1016/0003-9861(70)90408-x